BioSpring
anh-huong-probiotic-bacillus-dang-bao-tu-chiu-nhiet-den-nang-suat-vi-khuan-va-hinh-thai-vi-the-bieu-mo-duong-ruot-ga-thit-long-mau

a??nh h?�a�Yng ca��a Probiotics Bacillus da??ng bA�o ta�� cha��u nhia��t A�a??n nA?ng sua??t, vi khua?�n vA� hA�nh thA?i vi tha�? bia�?u mA? A�?�a�?ng rua��t gA� tha��t lA?ng mA�u

21-12-2016

Pha??m Kim A?A?ng1*, NguyA?nA�A�A?A�nh TrA�nh2, Nguya��n HoA�ng Tha��nh1,

Nguya��n Tha�� Ph?�??ng Giang1A�vA� Nguya��n BA? Tia??p3A�

TA�M Ta?�T

A� A� A� A� A� NghiA?n ca��u A�A?nh giA? tA?c da�?ng ca��a cha?? pha?�m probiotics NeoAvi GroMax cha��aA�BacillusA�da??ng bA�o ta�� A�a??n kha?? nA?ng sa??n xua??t gA� tha��t, gia��ng Ri Ninh HoA�. Sau 13 tua?�n, cA?c cha�� tiA?u kha��i l?�a�?ng c?? tha�?, tA?ng tra�?ng trung bA�nh/ngA�y; thu nha?�n tha��c A?n/ngA�y ca��a gA� A�?�a�?c ba�� sung cha?? pha?�m NeoAvi GroMax (tra��n va��i ta�� la�� 0,3%) vA� gA� A�?�a�?c ba�� sung khA?ng sinh BMD (Bacitracin Methylene-Disalicylate) 10% (0,4g/kg tha��c A?n) cao h??n ca��a nhA?m A�a��i cha��ng (A�?�a�?c nuA?i ba?�ng kha?�u pha?�n c?? sa�Y). Ta�� la�� chuya�?n hA?a tha��c A?n, tiA?u ta��n tha��c A?n/kg tA?ng tra�?ng ca��a gA� A�?�a�?c ba�� sung cha?? pha?�m hoa?�c khA?ng sinh tha??p h??n ca��a gA� nhA?m A�a��i cha��ng. KhA?ng cA? sa�� sai khA?c kha��i l?�a�?ng vA� ma��t sa�� cha�� tiA?u cha??t l?�a�?ng tha��t gia�?a cA?c lA? gA� thA� nghia��m. Cha?? pha?�m NeoAvi GroMax lA�m gia??m sa�� l?�a�?ng vi khua?�nA�E.coliA�vA�A�SalmonellaA�sp. trong ha��i trA�ng vA� trong phA?n; lA�m tA?ng sa�� l?�a�?ngA�LactobacillusA�sp.A�trong ha��i trA�ng. KhA?ng sinh lA�m gia??m sa�� l?�a�?ngA�LactobacillusA�sp. trong manh trA�ng gA� thA� nghia��m. Chia�?u cao vA� chia�?u dA�y lA?ng nhung bia�?u mA? tA? trA�ng vA� khA?ng trA�ng tA?ng lA?n d?�a��i tA?c da�?ng ca��a cha?? pha?�m. NghiA?n ca��u A�A? cha��ng minh A�?�a�?c tA?c da�?ng ca��a probiotics da??ng bA�o ta�� cha��u nhia��t A�a??n nA?ng sua??t gA� tha��t thA?ng qua tA?c da�?ng ca??i thia��n ha�� vi sinh va?�t A�?�a�?ng rua��t vA� sa��c kha�?e bia�?u mA? rua��t.

Ta�� khA?a: GA� tha��t, nA?ng sua??t,A�NeoAvi GroMax, Ri Ninh HA?a

A�

ABSTRACT

Effect of heat resistance Bacillus spores as a probiotics feed supplement on the performance, intestinal microbiota composition and epithelial villum measurements of colored broilers

Pham Kim Dang, Nguyen Dinh Trinh, Nguyen Hoang Thinh,

Nguyen Thi Phuong Giang and Nguyen Ba Tiep

A� A� A� A� The study aimed to assess effects of NeoAvi GroMax, a probiotics containing Bacillus spores, on performance of Ninh Hoa colored broilers breed. Final body weight, ADG, DFI after 13 weeks trial of chickens fed on basal diet supplemented with NeoAvi GroMax (0,3% supplement) and chickens fed on basal diet supplemented with BMD (Bacitracin Methylene-Disalicylate) 10% (0.4g/kg feed) were higher than that of control chickens (given basal diet only).A� Both the probiotics and antibiotic supplement led to lower FCR and feed weight per kg BW gain. Carcass characteristics and meat organoleptic quality were non-significant different among groups. NeoAvi GroMax supplement resulted in lower jejunum and fecalA�E. coliA�andA�SalmonellaA�sp. counts; higher caecalA�LactobacillusA�count. In contract, BMD caused the decrease of cecalA�LactobacillusA�count. Duodenum and Jejunum villi height and thickness were higher in the chickens fed on probiotics-supplemented diet. The results proved productive effects of heat resistanceA�BacillusA�spores in chicken with positive effects on intestinal microbiota and epithelial cells.

Keywords:A�NeoAvi GroMax, Performance, Broiler, Ri Ninh Hoa breed

1. A?a?�T Va?�N A?a��

A� A� A� A� Trong chA?n nuA?i, A�a?�c bia��t lA� chA?n nuA?i cA?ng nghia��p, khA?ng sinh A�A?ng vai trA? quan tra�?ng khA?ng cha�� trong phA?ng tra�� ba��nh mA� cA?n A�?�a�?c sa�� da�?ng nh?� cha??t kA�ch thA�ch sinh tr?�a�Yng ca��a va?�t nuA?i. Ta�� nha�?ng nA?m 50, khA?ng sinh A�A? A�?�a�?c sa�� da�?ng nha?�m kA�ch thA�ch sinh tr?�a�Yng trong chA?n nuA?i la�?n vA� gia ca?�m. Tuy nhiA?n, sa�� da�?ng khA?ng A�A?ng nguyA?n ta??c vA� la??m da�?ngA� khA?ng sinh A�A? vA� A�ang lA�m gia??m tA?c da�?ng phA?ng vA� tra�� ba��nh ca��a khA?ng sinh,A� a??nh h?�a�Yng A�a??n sa��c kha�?e ca��ng A�a��ng. ChA�nh vA� tha??, ta�� thA?ng 1 nA?m 2006, a��y ban ChA?u A�u A�A? ca??m sa�� da�?ng khA?ng sinh nha?�m ma�?c A�A�ch kA�ch thA�ch sinh tr?�a�Yng (cha�� tha�� 1831/2003/EEC). Trong tha?�p ka�� qua, A�A? cA? nhia�?u nghiA?n ca��u ta?�p trung vA�o tA�m kia??m cA?c ph?�??ng phA?p thay tha?? sa�� da�?ng khA?ng sinh cA�ng nh?� tA�m kia??m cA?c cha??t va��a cA? kha?? nA?ng kA�ch thA�ch sinh tr?�a�Yng, an toA�n, thA?n thia��n A�a�? gia??m nguy c?? ma??c ba��nh A�?�a�?ng rua��t cho va?�t nuA?i vA� ca??i thia��n cha??t l?�a�?ng sa??n pha?�m cho ng?�a�?i tiA?u dA?ng nh?� cha?? pha?�m probiotics, prebiotic, symbiotic, enzymes, axit ha�?u c?? vA� cA?c cha??t pha�? gia khA?c A�A? vA� A�ang A�?�a�?c phA?t tria�?n (FlutonA�et al., 2002). TA?c da�?ng tA�ch ca��c ca��a probiotics A�?�a�?c cha��ng minh thA?ng qua kha?? nA?ng kA�ch thA�ch tA�nh thA?m A?n (Nahashon et al., 1994), ca??i thia��n, thia??t la?�p cA?n ba?�ng ha�� vi sinh va?�t A�?�a�?ng rua��t (Fuller, 1989), ca??i thia��n cha��c nA?ng tiA?u hA?a (Collins et al., 1999). NgoA�i ra, probiotics kA�ch thA�ch ha�� mia��n da��ch (Perdigon et al., 1999; Collins et al., 1999). VA� va?�y, trong chA?n nuA?i, A�a?�c bia��t chA?n nuA?i cA?ng nghia��p, sa�� da�?ng probiotics A�a�? ca??i thia��n sa��c kha�?e A�?�a�?ng rua��t qua A�A? ca??i thia��n sa��c kha�?e vA� sa��c sa??n xua??t ca��a va?�t nuA?i (Block et al.,2002). Tuy nhiA?n, va?�n cA?n cA? nha�?ng tranh lua?�n va�? tA?c da�?ng ca��a probiotics. NghiA?n ca��u ca��a Maxwell vA� ca��ng sa�� (1983);A� Hong vA� ca��ng sa�� (2002) cho ra?�ng ba�� sung probiotics cha��aA�LactobacillusA�sp. cA? tA?c A�a��ng ta��t A�a??n kha?? nA?ng tiA?u hA?a. Ma��t sa�� nghiA?n ca��u khA?c la??i cho ra?�ng ba�� sung probiotics cA? cha��aA�LactobacillusA�hoa?�cA�BacillusA�khA?ng a??nh h?�a�Yng A�a??n kha?? nA?ng tiA?u hA?a ca��a la�?n thA� nghia��m (Hale et al., 1979, Kornegay et al., 1996). Nha�?ng ka??t lua?�n trA?i ng?�a�?c nA�y cA? tha�? do cA? sa�� khA?c nhau va�? A�a?�c tA�nh ca��a vi sinh va?�t trong cha?? pha?�m,A� A�ia�?u kia��n ba??o qua??n, vA� cA?ch tha��c sa�� da�?ng cha?? pha?�m probiotics.

A� A� A� A� a�z Via��t Nam, sa�� da�?ng khA?ng sinh trong tha��c A?n chA?n nuA?i A�ang ngA�y cA�ng A�?�a�?c qua??n lA? cha?�t cha??. A?a??n 15 thA?ng 7 nA?m 2016, sa�� l?�a�?ng cA?c loa??i khA?ng sinh A�?�a�?c cho phA�p cA? ma?�t trong tha��c A?n cha�� cA?n 15 loa??i (Ba�� NN&PTNT, 2016) vA� sa?? ca??m hoA�n toA�n vA�o nA?m 2018. Do A�A?, sa�� da�?ng probiotics ngA�y cA�ng A�?�a�?c cA?c nhA� khoa ha�?c, c?? quan qua??n lA? vA� ng?�a�?i chA?n nuA?i quan tA?m. Hia��n nay trA?n tha�� tr?�a�?ng cA? nhia�?u sa??n pha?�m probiotics nh?�ng A�a sa�� sa�� da�?ng ba?�ng cA?ch A�?�a tra��c tia??p vA�o tha��c A?n hoa?�c n?�a��c ua��ng. NeoAvi GroMax lA� ma��t trong nha�?ng sa??n pha?�m ma��i cha��a bA�o ta�� vi khua?�n cA? kha?? nA?ng cha��u nhia��t ca��a cA?ng ty BioSpring va��i ?�u A�ia�?m cA? tha�? sa�� da�?ng tra��n vA�o tha��c A?n trong cA?c nhA� mA?y sa??n xua??t tha��c A?n cA?ng nghia��p. NghiA?n ca��u nA�y A�?�a�?c tha��c hia��n nha?�m A�A?nh giA? a??nh h?�a�Yng ca��a cha?? pha?�m NeoAvi GroMax A�a��i va��i gA� tha��t, lA�m c?? sa�Y khuya??n cA?o sa�� da�?ng cho cA?c nhA� sa??n xua??t tha��c A?n vA� ng?�a�?i chA?n nuA?i.

2.Va?�T LIa��U VA� PH???�NG PHA?P NGHIASN Ca�?U

2.1. A?a��a A�ia�?m vA� tha�?i gian thA� nghia��m

ThA� nghia��m A�?�a�?c tha��c hia��n ta??i Tra??i tha��c nghia��m, Khoa ChA?n nuA?i, Ha�?c via��n NA?ng nghia��p Via��t Nam ta�� thA?ng 9 A�a??n thA?ng 12 nA?m 2015.

2.2. Ba�� trA� thA� nghia��m

A� A� A� A� ThA� nghia��m bao ga��m 180 gA� tha��t lA?ng mA�u, gia��ng Ri Ninh HA?a, 2 tua?�n tua��i A�?�a�?c sa�� da�?ng trong 3 A�a�?t thA� nghia��m. Ma��i A�a�?t, 60 gA� A�?�a�?c phA?n nga?�u nhiA?n thA�nh 3 nhA?m (10 gA� tra��ngA� vA� 10 gA� mA?i/nhA?m). GA� nhA?m A�a��i cha��ng A�?�a�?c nuA?i ba?�ng kha?�u pha?�n c?? sa�Y (khA?ng A�?�a�?c ba�� sung khA?ng sinh vA� probiotics). GA� nhA?m 2 A�?�a�?c cho A?n kha?�u pha?�n c?? sa�Y cA? ba�� sung BMD (Bacitracin Methylene-Disalicylate) 10% va��i va��i l?�a�?ng 0,4 g/kg tha��c A?n. GA� nhA?m 3 A�?�a�?c cho A?n kha?�u pha?�n c?? sa�Y cA? ba�� sung NeoAvi GroMax 0,3%. Ta??t ca?? gA� thua��c 3 ba nhA?m A�?�a�?c phA?ng ca?�u trA?ng ba?�ng Diclazuril 0,5% va��i lia�?u 0,25g/kg a�Y giai A�oa??n tr?�a��c 5 tua?�n tua��i. Kha?�u pha?�n A?n A�?�a�?c cA?n A�a��i trA?n c?? sa�Y 2 kha?�u pha?�n A?n dA�nh cho hai giai A�oa??n ca��a gA� tha��t lA?ng mA�u ta�� 1 A�a??n 28 ngA�y tua��i (DM 87,93%, Protein 17,96%, ME 3139 Kcal/kg, Ash 3,30%) vA� ta�� 29 ngA�y A�a??n xua??t bA?n (DM 88,22%, Protein 19,7%, ME 3069 Kcal/kg, Ash 3,49%). CA?ng tha��c tha��c A?n sa�� da�?ng cho nhA?m A�a��i cha��ng (kha?�u pha?�n c?? sa�Y) lA� cA?ng tha��c tha��c A?n A�?�a�?c ca?�n A�a��i la??i ta�� cA?ng tha��c tha��c A?n cA? cha��a BMD (mA? tha��c A?n dA?ng cho gA� tha��t lA?ng mA�u A�ang A�?�a�?c th?�??ng ma??i trA?n tha��c ta??). CA?n tha��c A?n dA?ng cho nhA?m 3 A�?�a�?c xA?y da��ng trA?n c?? sa�Y kha?�u pha?�n c?? sa�Y ba�� sung NeoAvi GroMax 0,3%.

2.3. Ph?�??ng phA?p A�A?nh giA? cA?c cha�� tiA?u

A� A� A� A� CA?c cha�� tiA?u nA?ng sua??t A�?�a�?c A�A?nh giA? theo BA?i Ha�?u A?oA�n vA� ctv (2011). Kha��i l?�a�?ng gA� A�?�a�?c xA?c A�a��nh hA�ng tua?�n ba?�ng cA?n A�a��ng ha�� loa??i 5kg. Ha�� sa�� chuya�?n hA?a tha��c A?n A�?�a�?c tA�nh toA?n da��a trA?n l?�a�?ng tha��c A?n thu nha?�n hA�ng ngA�y.

A� A� A� A� Khi gA� A�?�a�?c 15 tua?�n tua��i, 4 gA�/nhA?m (2 tra��ng, 2 mA?i) A�?�a�?c ma�� kha??o sA?t A�a�? A�A?nh giA? kha?? nA?ng cho tha��t vA� ma��t sa�� cha�� tiA?u cha??t l?�a�?ng ca??m quan. pH c?? l?�a�?n A�?�a�?c A�o a�Y tha�?i A�ia�?m 15 phA?t vA� 24 gia�? sau gia??t ma�� ba?�ng mA?y A�o pH mettre Testo 230 (Germany).

A� A� A� A� Sau gia??t ma��, ma?�u c?? A�A?i vA� c?? l?�a�?n A�?�a�?c la??y A�a�? A�A?nh giA? A�a�� ma�?m ba?�ng thia??t ba�� Warner – Bratzler 2000D. MA�u sa??c tha��t A�?�a�?c xA?c A�a��nh ba?�ng mA?y Minolta CR-410 vA� A�?�a�?c tha�? hia��n thA?ng qua tham sa�� L*- (lightness), a*-(redness) and b*- (yellowness).

A� A� A� A� CA?c ma?�u cha??t cha��a trong ha��i trA�ng, manh trA�ng vA� phA?n A�?�a�?c la??y A�a�? xA?c A�a��nh ma?�t A�a�� (Log10A�CFU/g) ca��a ma��t sa�� vi khua?�nA�E.coli, SalmonellaA�sp., Clostridium perfringensA�vA�A�LactobacillusA�sp.A�CA?c tiA?u chua?�n tham chia??u t?�??ng a��ng va��i cA?c vi khua?�n trA?n ga��m ISO 13349/2001, ISO 6579/2003, ISO 7937/2004 vA� ISO/Dis 11290/1994.

A� A� A� A� TiA?u ba??n vi tha�? ma?�u ha��i trA�ng vA� manh trA�ng (trong formalin 10%) A�?�a�?c lA�m theo quy trA�nh chua?�n. HA�nh thA?i bia�?u mA? rua��t trA?n tiA?u ba??n nhua��m HE A�?�a�?c quan sA?t va��i kA�nh hia�?n vi Kniss MBL-2000T (Olympus, Japan). Chia�?u cao vA� chia�?u dA�y lA?ng nhung bia�?u mA? rua��t A�?�a�?c A�o ba?�ng pha?�n ma�?m Infinity Analysis.

2.4. Ph?�??ng phA?p phA?n tA�ch sa�� lia��u

A� A� A� A� Ta??t ca?? sa�� lia��u thA� nghia��m A�?�a�?c phA?n tA�ch ba?�ng pha?�n ma�?m tha��ng kA? SAS 9.1. Sai khA?c cA? A? nghA�a A�?�a�?c xA?c A�a��nh ba?�ng Duncana��s Multiple Range Test.

3.Ka??T QUa?? VA� THa??O LUa?�N

3.1. a??nh h?�a�Yng cha?? pha?�m Neovia GroMax A�a??n nA?ng sua??t gA� tha��t

A� A� A� A� Kha?? nA?ng sa??n xua??t gA� tha��t tha�? hia��n qua cA?c tham sa�� quan tra�?ng nh?� kha?? nA?ng tA?ng kha��i l?�a�?ng vA� tiA?u ta��n tha��c A?n. CA?n ca�� vA�o cA?c cha�� tiA?u nA�y A�A?nh giA? A�?�a�?c hia��u qua?? chA?n nuA?i. Theo Chambers vA� ctv (1984) , ha�� sa�� t?�??ng quan gia�?a kha��i l?�a�?ng c?? tha�? vA� tA?ng tra�?ng va��i tiA?u ta��n tha��c A?n th?�a�?ng ra??t cao (r = 0,5- 0,9), gia�?a sinh tr?�a�Yng vA� chuya�?n hA?a tha��c A?n cA? sa�� t?�??ng quan A?m (r= -0,2A� -0,8). Ka??t qua?? tha�� nghia��m vA� so sA?nh tha��ng kA? ta��ng ha�?p a�Y ba??ng 2 cho tha??y khA?ng cA? sa�� sai khA?c tha��ng kA? gia�?a cA?c cha�� tiA?u va�? kha?? nA?ng tA?ng tra�?ng bA�nh quA?n ngA�y, kha?? nA?ng thu nha?�n tha��c A?n hA�ng ngA�y vA� ha�� sa�� chuya�?n hA?a tha��c A?n/kg kha��i l?�a�?ng gia�?a hai lA? ba�� sung khA?ng sinh vA� lA? ba�� sung cha?? pha?�m Neovia GroMax (P>0,05). Tuy nhiA?n, cA?c cha�� tiA?u nA�y ca��a gA� thA� nghia��m a�Y lA? ba�� sung khA?ng sinh vA� ba�� sung cha?? pha?�m Neovia GroMax A�?�a�?c ca??i thia��n h??n so va��i lA? A�a��i cha��ng vA� sa�� sai khA?c cA? A? nghA�a thA?ng kA? (P<0,05). Kha?? nA?ng tA?ng kha��i l?�a�?ng bA�nh quA?n ngA�y ca��a lA? ba�� sung khA?ng sinh vA� lA? ba�� sung cha?? pha?�m Neovia Gromax la?�n l?�a�?t lA� 19,79 vA� 20,14 gam/com/ngA�y, cao h??n lA? A�a��i cha��ng cha�� A�a??t 19,00 gam/con/ngA�y. Tuy nhiA?n, giA? tra�� FCR ca��a lA? A�a��i cha��ng la??i cao h??n so va��i giA? tra�� nA�y ca��a hai lA? thA� nghia��m. TiA?u ta��n tha��c A?n bA�nh quA?n cho ma��t kg tA?ng kha��i l?�a�?ng ca��a lA? A�a��i cha��ng lA� 3,64 kg trong khi A�A? lA? ba�� sung khA?ng sinh vA� lA? ba�� sung cha?? pha?�m Neovia GroMax cha�� tiA?u ta��n la?�n l?�a�?t lA� 3,45 vA� 3,46 kg tha��c A?n.

anh-huong-cua-probiotic-bacillusdang-bao-tu-chiu-nhiet-den-nang-suat-vi-khuan-va-hinh-thai-vi-the-bieu-mo-duong-ruot-ga-thit-long-mau

3.2. a??nh h?�a�Yng ca��a ba�� sung cha?? pha?�m Neovia GroMax A�a??n nA?ng sua??t vA� cha??t l?�a�?ng tha��t gA�

A� A� A� A� Ka??t qua?? kha??o sA?t kha?? nA?ng cho tha��t vA� ma��t sa�� cha�� tiA?u ca??m quan va�? cha??t l?�a�?ng tha��t cho tha??y, ta??t ca?? cA?c cha�� tiA?u nA?ng sua??t vA� cha??t l?�a�?ng tha��t khA?ng cA? sa�� sai khA?c gia�?a cA?c lA? A�a��i cha��ng vA� thA� nghia��m (P>0,5). Kha��i l?�a�?ng thA?n tha��t, kha��i l?�a�?ng tha��t A�A?i vA� l?�a�?n a�Y lA? ba�� sung cha?? pha?�m vA� lA? ba�� sung khA?ng sinh cA? xu h?�a��ng cao h??n so va��i lA? A�a��i cha��ng, tuy nhiA?n sa�� sai khA?c khA?ng cA? A? nghA�a tha��ng kA?.

A� A� A� A� T?�??ng ta��, cA?c cha�� sa�� liA?n quan A�a??n ca??m quan nh?� pH, cA?c tham sa�� mA�u sa??c tha��t (L*, a* vA� b*) vA� A�a�� dai ca��a tha��t cA�ng khA?ng cA? sa�� khA?c bia��t cA? A? nghA�a tha��ng kA? (P>0,05).

anh-huong-cua-probiotic-bacillusdang-bao-tu-chiu-nhiet-den-nang-suat-vi-khuan-va-hinh-thai-vi-the-bieu-mo-duong-ruot-ga-thit-long-mau

A� A� A� A� NghiA?n ca��u nA�y cho ka??t qua?? kha��i l?�a�?ng sa��ng, kha��i l?�a�?ng gia??t tha��t ca��a ca?? 3 nhA?m gA� cao h??n so va��i ma��t sa�� gia��ng gA� khA?c; ca�? tha�? nh?� GA� Ri a�Y 14 tua?�n tua��i cA? kha��i l?�a�?ng sa��ng lA� 1,055kg, kha��i l?�a�?ng gia??t tha��t lA� 0,822 kg, kha��i l?�a�?ng thA?n tha��t lA� 0,738kg; gA� Ha��MA?ng cA? kha��i l?�a�?ng sa��ng lA� 1,125kg, kha��i l?�a�?ng gia??t tha��t lA� 0,817kg vA� kha��i l?�a�?ng thA?n tha��t lA� 0,747kg (LA? Tha�� ThuA? vA� ctv, 2010); cao h??n kha��i l?�a�?ng c?? tha�? lA?c 14 tua?�n tua��i ca��a gA� Ri vA� con lai (GA� Ri-Sasso-L?�??ng Ph?�a�?ng) lA� 1,240kg vA� gA� Ri lA� 1,066kg (Nguya��n BA? MA?i vA� Pha??m Kim A?A?ng, 2016) nh?�ng la??i tha??p h??n so va��i gia��ng gA� khA?c nh?� gA� L?�??ng Ph?�a�?ng a�Y 12 tua?�n tua��i A�A? A�a??t 2,0-2,57kg/con (NguyA?I?n Huy A?aI?t vaI� NguyA?I?n ThaI�nh A?A?I�ng, 2000).

A� A� A� A� Nh?� va?�y, cA?c ka??t qua?? va�? kha??o sA?t nA?ng sua??t tha��t ca��a gA� qua nghiA?n ca��u A�a�?u na?�m trong gia��i ha??n ca��a cA?c gia��ng gA� a�Y Via��t Nam.

3.3. a??nh h?�a�Yng ca��a Neovia GroMax A�a??n ma?�t A�a�� ma��t sa�� vi khua?�n A�?�a�?ng rua��t gA�

A� A� A� A� Ta��ng sa�� vi khua?�n trong cha??t cha��a ha��i trA�ng, cha??t cha��a manh trA�ng vA� trong phA?n ca��a ba lA? gA� thA� nghia��m (ba??ng 3) lA� ma��t trong nha�?ng cA?n ca�� xA?c A�a��nh tA?c da�?ng cA? la�?i ca��a cha?? pha?�m.

anh-huong-cua-probiotic-bacillusdang-bao-tu-chiu-nhiet-den-nang-suat-vi-khuan-va-hinh-thai-vi-the-bieu-mo-duong-ruot-ga-thit-long-mau

A� A� A� A� Ta��ng sa��A�E.coliA�vA�A�SalmonellaA�sp. trong cha??t cha��a ha��i trA�ng ca��a gA� A�?�a�?c ba�� sung Neovia ProMax tha??p h??n ta��ng sa��A�E.coliA�ta�� cA?c ma?�u cha??t cha��a ha��i trA�ng ca��a gA� A�?�a�?c nuA?i ba?�ng kha?�u pha?�n c?? sa�Y vA� nhA?m A�?�a�?c ba�� sung khA?ng sinh (P<0,05). Ta�� la�� gia??m ta��ng sa��A�E.coliA�trong ha��i trA�ng do tA?c A�a��ng ca��a cha?? pha?�m lA� 35,66% so va��i A�a��i cha��ng (t?�??ng a��ng lA� 3,32.106A�vA� 5,16.106A�tria��u/g cha??t cha��a). Va��i ma?�u cha??t cha��a manh trA�ng, khA?ng cA? sa�� khA?c nhau va�? sa�� l?�a�?ngA�E.coliA�a�Y ca?? 3 nhA?m thA� nghia��m (P>0,05). Ta��ng sa��A�E.coliA�trong phA?n gA� A�?�a�?c ba�� sung khA?ng sinh tha??p h??n so va��i hai lA? cA?n la??i. NeoAvia GroMax lA�m gia??m 58,7% sa�� l?�a�?ngA�SalmonellaA�sp. trong ha��i trA�ng vA� 36,4% trong phA?n so va��i A�a��i cha��ng. Ta��ng sa��A�C.perfringensA�trong ca?? 3 loa??i ma?�u cha??t cha��a ha��i trA�ng, manh trA�ng vA� trong phA?n khA?ng khA?c nhau a�Y ca?? ba nhA?m gA� (P>0,05). Sa�� l?�a�?ngA�LactobacillusA�sp. tA?ng 23% trong cha??t cha��a ha��i trA�ng ca��a gA� A�?�a�?c ba�� sung Neovia GroMax nh?�ng khA?ng lA�m thay A�a��i sa�� l?�a�?ng vi khua?�n nA�y trong manh trA�ng vA� trong phA?n. KhA?ng sinh khA?ng a??nh h?�a�Yng A�a??n ta��ng sa��A�LactobacillusA�trong ha��i trA�ng vA� trong phA?n so va��i A�a��i cha��ng nh?�ng lA�m gia??m sa�� l?�a�?ng vi khua?�n nA�y trong manh trA�ng (P<0,05). Sa�� l?�a�?ngA�LactobacillusA�trong mang trA�ng gA� ba�� sung khA?ng sinh tha??p h??n 13,9% sa�� l?�a�?ng vi khuA?n nA�y trong manh trA�ng gA� A�?�a�?c ba�� sung cha?? pha?�m probiotics. Nh?� va?�y NeoAvi GroMax tA?c A�a��ng tA�ch ca��c A�a??n ha�� vi sinh va?�t trong ha��i trA�ng (tA?ng sa�� l?�a�?ngA�LactobacillusA�sp; gia??m sa�� l?�a�?ngA�E.coliA�vA�A�Salmonella sp); vA� gia??m sa�� l?�a�?ngA�SalmonellaA�sp. trong phA?n. KhA?ng sinh khA?ng tA?c A�a��ng rA� ra��t A�a??nA�E.coliA�vA�A�SalmonellaA�sp. trong ha��i trA�ng vA� manh trA�ng nh?�ng lA�m gia??m sa�� l?�a�?ngA�SalmonellaA�trong phA?n. Tuy nhiA?n, ma��t A�ia�?u ca?�n A�?�a�?c quan tA?m lA� khA?ng sinh lA�m gia??m sa�� l?�a�?ngA�LactobacillusA�- ma��t vi khua?�n cA? la�?i trong manh trA�ng.

A� A� A� A� Chen vA� ctv (2013) cho bia??tA�Bacillus subtilisA�vA� na??m menA�SaccharomycesA�cerevisiaeA�trong tha��c A?n cA? tA?c da�?ng lA�m tA?ng sa�� l?�a�?ngA�LactobacillusA�sp. vA� a��c cha??A�E.coliA�trong rua��t non vA� rua��t giA�. A?a?�c bia��t, probiotics cha��aA�L. acidophilusA�lA�m tA?ng sa�� l?�a�?ngA�LactobacillusA�sp.A� trong manh trA�ng ca��a gA� tha��t. NghiA?n ca��u ca��a Li et al. (2014) cho tha??y probiotics cha��aA�L. acidophilusA�lA�m giA�m sa�� l?�a�?ngA�SaccharomycesA�vA� tA?ng sa�� l?�a�?ngA�LactobacillusA�trong manh trA�ng. Trong qua?�n tha�? vi sinh va?�t ca��a rua��t,A�L.acidophilusA�ta??o mA?i tr?�a�?ng pH tha??p ta�� A�A? a��c cha?? sa�� phA?t tria�?n ca��a vi khua?�n vA� na??m gA?y ba��nh. ThA?m vA�o A�A?,A�L.acidophilusA�cA? kha?? nA?ng thA�ch nghi va��i mA?i tr?�a�?ng rua��t vA� kA�ch thA�ch cA?cA�LactobacillusA�trong manh trA�ng ca��a gA� A�?�a�?c ba�� sungA�L. acidophilusA�(JinA�et al, 1996).A�L. acidophilusA�cA? tA?c da�?ng tA�ch ca��c va��i cA?n ba?�ng vi sinh va?�t A�?�a�?ng rua��t (ZhangA�et al, 2004).

A� A� A� A� TA?c da�?ng ca��a NeoAvi GroMax lA�m tA?ng kha��i l?�a�?ng c?? tha�? vA� cha�� sa�� tA?ng tra�?ng trung bA�nh ngA�y tha��ng nha??t va��i ma��t sa�� nghiA?n ca��u A�A? A�?�a�?c cA?ng ba�� va��i probiotics cha��aA�Bacillus subtilisA�(KhaksefidiA�et al., 2006). LiuA�et al. (2007) ba�� sung vA�o kha?�u pha?�n c?? sa�Y cha?? pha?�m cha��aA�Lactobacillus reuteriA�ca??i thia��n tA?ng tra�?ng gA� tha��t. Mountzouris vA� ctv (2007) cha��ng minh cA?c cha��ngA�Pediococcus,A�Enterococcus,A�LactobacillusA�vA�A�BifidobacteriumA�ba�� sung trong tha��c A?n, n?�a��c ua��ng cho gA� tha��t lA�m gia??m tra�� sa�� FCR. Ka??t qua?? nghiA?n ca��u nA�y cho tha??yA�NeoAvi GromaxA�cA? xu h?�a��ng tA?c da�?ng ta��t, t?�??ng ta�� nh?� nhia�?u cha?? pha?�m cha��a vi sinh va?�t cA? la�?i A�A? A�?�a�?c cA?c nghiA?n ca��u tr?�a��c A�A?y xA?c A�a��nh.

A� A� A� A� TA?c da�?ng ca��a probiotics cA? tha�? do kha?? nA?ng A�ia�?u hA?a mia��n da��ch vA� ha�� tra�? cA?c vi sinh va?�t cA? la�?i trong ha�� vi sinh va?�t A�?�a�?ng rua��t, ca??i thia��n kha?? nA?ng bia??n A�a��i tha��c A?n vA� ha??p thu cha??t dinh d?�a�?ng ca��a va?�t cha�� (Mountzouris et al., 2010; Alkhalf et al., 2010).

3.4. a??nh h?�a�Yng ca��a ba�� sung cha?? pha?�m Neovia GroMax A�a??n hA�nh thA?i vA� kA�ch th?�a��c lA?ng nhung bia�?u mA? niA?m ma??c rua��t gA� thA� nghia��m

A� A� A� A� A?a sa�� la�� nhung bia�?u mA? tA? trA�ng ca��a gA� A�?�a�?c ba�� sung NeoAvi GroMax cA? pha?�n A�a��nh nguyA?n va??n (hA�nh 1c). Ra??t nhia�?u lA?ng nhung bia�?u mA? tA? trA�ng ca��a gA� A�a��i cha��ng (hA�nh 1a) vA� gA� A�?�a�?c ba�� sung khA?ng sinh (hA�nh 1b) cA? pha?�n A�a��nh khA?ng nguyA?n va??n. Ranh gia��i gia�?a cA?c lA?ng nhung ca��a gA� A�?�a�?c ba�� sung NeoAvi GroMax cA�ng rA� h??n. Bia�?u mA? khA?ng trA�ng ca��a gA� A�?�a�?c ba�� sung NeoAvi GroMax (hA�nh 2c) cA�ng cA? tra??ng thA?i ta��t h??n ca��a gA� A�a��i cha��ng (hA�nh 2a) vA� gA� A�?�a�?c ba�� sung khA?ng sinh (hA�nh 2b).

anh-huong-cua-probiotic-bacillusdang-bao-tu-chiu-nhiet-den-nang-suat-vi-khuan-va-hinh-thai-vi-the-bieu-mo-duong-ruot-ga-thit-long-mau

anh-huong-cua-probiotic-bacillusdang-bao-tu-chiu-nhiet-den-nang-suat-vi-khuan-va-hinh-thai-vi-the-bieu-mo-duong-ruot-ga-thit-long-mau

A� A� A� A� Chia�?u cao vA� chia�?u dA�y lA?ng nhung bia�?u mA? tA? trA�ng vA� khA?ng trA�ng A�?�a�?c trA�nh bA�y a�Y ba??ng 4.

anh-huong-cua-probiotic-bacillusdang-bao-tu-chiu-nhiet-den-nang-suat-vi-khuan-va-hinh-thai-vi-the-bieu-mo-duong-ruot-ga-thit-long-mau

A� A� A� A� Cha?? pha?�m Neovia GroMax lA�m tA?ng chia�?u cao vA� chia�?u dA�y lA?ng nhung tA? trA�ng vA� khA?ng trA�ng so va��i lA? A�a��i cha��ng (cho A?n kha?�u pha?�n c?? sa�Y) vA� lA? ba�� sung khA?ng sinh. Ta�� la�� tA?ng t?�??ng a��ng do tA?c da�?ng ca��a cha?? pha?�m so va��i A�a��i cha��ng lA� 30% (1.322mm a�Y lA? A�?�a�?c ba�� sung Neovia GroMax vA� 1,015mm a�Y lA? A�a��i cha��ng) vA� 20% (0,316mm so va��i 0,239mm). Chia�?u cao lA?ng nh?�ng khA?ng trA�ng cA�ng tA?ng 20% so va��i A�a��i cha��ng (0,878mm so va��i 0,731mm). KhA?ng sinh khA?ng lA�m thay A�a��i kA�ch th?�a��c lA?ng nhung bia�?u mA? so va��i A�a��i cha��ng.

A� A� A� A� Dia��n tA�ch ba�? ma?�t bia�?u mA? rua��t t?�??ng quan thua?�n va��i kha?? nA?ng bia??n A�a��i tha��c A?n vA� A�a?�c bia��t lA� kha?? nA?ng ha??p thu dinh d?�a�?ng. Ba�? ma?�t bia�?u mA? cA? dia��n tA�ch cA�ng la��n va��i cA?c ta?? bA�o bia�?u mA? toA�n va??n lA� ya??u ta�� quan tra�?ng A�a??m ba??o kha?? nA?ng chuya�?n hA?a tha��c A?n. NghiA?n ca��u ca��a nhia�?u tA?c gia?? A�a�?u cho tha??y kha?? nA?ng chuya�?n hA?a tha��c A?n cha��u a??nh h?�a�Yng ca��a chia�?u cao cA?c lA?ng nhung vA� tA�nh toA�n va??n ca��a cA?c lA?ng nhung bia�?u mA? rua��t (ErfaniA�et al., 2013). Theo ChichlowskiA�et al. (2007), probiotics cha��aA�Lactobacilli bifidobacterium ther-mophilumA�vA�A�Enterococcus faeciumA�lA�m tA?ng chia�?u cao lA?ng nhung khA?ng trA�ng a�Y gA� tha��t trong khi khA?ng sinh salinomycin ba�� sung trong tha��c A?n khA?ng cA? tA?c da�?ng nA�y. Chia�?u cao ca��a lA?ng nhung cA?n liA?n quan A�a??n cA?c A�a��c ta�� (AwadA�et al., 2006). Nhia�?u cha?? pha?�m probiotics vA� symbiotic cA? tA?c da�?ng lA�m tA?ng chia�?u cao lA?ng nhung bia�?u mA? niA?m ma??c rua��t (ErfaniA�et al. 2013).

A� A� A� A� Chia�?u cao vA� chia�?u dA�y lA?ng nhung lA� hai cha�� sa�� chA�nh bia�?u hia��n tA�nh toA�n va??n ca��a lA?ng nhung vA� quya??t A�a��nh dia��n tA�ch ba�? ma?�t bia�?u mA? rua��t – ya??u ta�� a??nh h?�a�Yng A�a??n kha?? nA?ng ha??p thu cha??t dinh d?�a�?ng ca��a gA�. A?A?y chA�nh lA� ma��t trong hai c?? cha?? tA?c A�a��ng chA�nh lA�m gia??m FCR vA� tA?ng kha��i l?�a�?ng c?? tha�? ca��a gA� A�?�a�?c ba�� sung cha?? pha?�m Neovia GroMax va��i thA�nh pha?�n vi khua?�nA�BacillusA�da??ng bA�o ta��.

4.Ka??t lua?�n

A� A� A� A� Cha?? pha?�m Neovia GroMax cha��a bA�o ta��A�BacillusA�cha��u nhia��t cA? tA?c da�?ng ca??i thia��n kha��i l?�a�?ng c?? tha�?, tA?ng tra�?ng trung bA�nh/ngA�y, tiA?u ta��n tha��c A?n/kg tA?ng tra�?ng vA� ta�� la�� chuya�?n hA?a tha��c A?n a�? gA� tha��t lA?ng mA�u gia��ng Ri Ninh HoA�.

A� A� A� A� Ba�� sung NeoAvi GroMax lA�m gia??m sa�� l?�a�?ng vi khua?�nA�E.coliA�vA�A�SalmonellaA�sp; tA?ng sa�� l?�a�?ngA�LactobacillusA�sp. Ta�� A�A? a??nh h?�a�Yng A�a??n ha�� vi sinh va?�t A�?�a�?ng rua��t, gia??m ta��ng sa�� vi khua?�n gA?y ba��nh vA� kA�ch thA�ch vi khua?�n cA? la�?i phA?t tria�?n, ca??i thia��n kha?? nA?ng tiA?u hA?a trong rua��t.

A� A� A� A� NeoAvi GroMax A�a??m ba??o tA�nh toA�n va??n ca��a lA?ng nhung bia�?u mA? rua��t, tA?ng chia�?u cao vA� chia�?u dA�y lA?ng nhung ta�� A�A? cA? tha�? lA�m tA?ng hia��u qua?? ha??p thu tha��c A?n vA� gia??m nguy c?? nhia��m ba��nh do vi khua?�n xA?m nha?�p qua niA?m ma??c rua��t.

TA�I LIa��U THAM KHa??O

  1. Alkhalf A, Alhaj M and Al-homidan IA�(2010). Influence of probiotic supplementation on blood parameters and growth performance in broiler chickens. Saudi J. Biol. Sci.., 17: 219-225.
  2. Awad, WA; BA�hm, J; Razzazi-Fazeli, E; Ghareeb, K and Zentek, JA�(2006). Effect of addition of a probiotic microorganism to broiler diets contaminated with deoxynivalenol on performance and histological alterations of intestinal villi of broiler chickens. Poult. Sci., 85: 974- 979.
  3. Blok, M. C., H. A. Vahl, L. de Lange, A. E. van de Braak, G. Hemke and M. Hessing. 2002. Nutrition and health of the gastrointestinal tract. Wageningen Academic. The Netherlands: 45-49.
  4. Ba�� NA?ng nghia��p & PhA?t tria�?n nA?ng thA?nA�(2016). ThA?ng t?� sa��A�06/2016/TT-BNNPTNT ngA�y 31A�thA?ngA�5A�nA?m 2016 va�? via��c hA�m l?�a�?ng khA?ng sinh A�?�a�?c phA�p sa�� da�?ng trong tha��c A?n chA?n nuA?i gia sA?c, gia ca?�m, nha?�m ma�?c A�A�ch kA�ch thA�ch sinh tr?�a�Yng.
  5. BA?i Ha�?u A?oA�n, Nguya��n Tha�� Mai, Nguya��n Thanh S??n, Nguya��n Huy A?a??tA�(2011). CA?c cha�� tiA?u dA?ng trong nghiA?n ca��u chA?n nuA?i gia ca?�m. NXB NA?ng nghia��p HA� Na��i.
  6. Chen W, Zhu XZ and Wang JP et alA�(2013). Effects of Bacillus subtilis var. natto andA�Saccharomyces cerevisiaeA�fermented liquid feed on growth performance, relative organ weight, intestinal microflora, and organ antioxidant status in Landes geese. J. Anim Sci., 91(2): 978-985.
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  10. Fluton, R, N,, Nersessian, B, N, and Reed, W, M,A�(2002), Prevention of Salmonella enteristidis infection in commercial duckling by oral chicken egg-derived antibody alone or in combination with probiotics Poultry Science Journal,A�81(34-40);
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Ngua��n: BA�i A�A?ng trA?n Ta??p chA� Khoa ha�?c Ka�? thua?�t chA?n nuA?i sa�� 213, thA?ng 11.2016